Metal pollution and its biological effects in swimming crab Portunus trituberculatus by NMR-based metabolomics

Yu, Deliang; Peng, Xiao; Ji, Chenglong; Li, Fei; Wu, Huifeng

Metal pollution and its biological effects in swimming crab Portunus trituberculatus by NMR-based metabolomics

2020

Yu, Deliang | Peng, Xiao | Ji, Chenglong | Li, Fei | Wu, Huifeng

Metal pollution in the Bohai Sea in China has posed a potential risk on marine organisms. In this work, crabs (Portunus trituberculatus) were sampled from four sites, namely a reference (site 3934) and three metal-polluted (sites 6151, 6351, and 3562) sites, located in the Bohai Sea. Metal concentrations in crab gill tissues were measured using inductively coupled plasma mass spectrometry. Cu, Zn, and Cd in crab samples from S3562 presented the highest concentrations. Particularly, Cu concentration exceeded the marine biological quality standard II. Cd contents in crab samples from all metal-polluted sites exceeded the marine biological quality standard I. Nuclear magnetic resonance-based metabolomics indicated metal pollution-induced immune stresses in crab samples from all metal-polluted sites. Metal pollution in S6151 and S6351 disturbed energy metabolism through differential pathways. For crab samples from S3562, the metabolic profile suggested that metal pollution mainly induced osmotic stress.

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