Multiple shoot induction and plant regeneration of Feronia limonia (L.) Swingle
2003
Hiregoudar, L.V. | Murthy, H.N. | Hema, B.P. | Hahn, E.J. | Paek, K.Y.
An in vitro regeneration system was developed for propagation of Feronia limonia (L.) Swingle. Axillary shoot proliferation was induced in the nodal segments on Murashige and Skoog (MS) medium supplemented with N6-benzylaminopurine/kinetin/thidiazuron (BAP/KN/TDZ). Maximum number of shoots (11.33 shoots per explant) were developed on a medium containing 5 μM BAP. Adventitious shoot regeneration occurred from leaf explants on MS media containing BAP/TDZ. The best response in terms of frequency of shoot regeneration (100%) and number of shoots per explant (7.33 shoots per explant) was observed on medium supplemented with 5 μM BAP. A proliferating shoot culture was established by repeatedly sub-culturing the original nodal and leaf explants on shoot multiplication medium after each harvest of newly formed shoots. Thus, 20–32 shoots were obtained in 5 months from a single nodal or leaf explant. Rooting was induced in 83.3% of the regenerated shoots on a half-strength MS medium supplemented with 1 μM α-naphthaleneacetic acid (NAA). The plantlets were acclimatized and established in soil.
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