Behavioral effects of titanium dioxide nanoparticles on larval zebrafish (Danio rerio)

Chen, Te-Hao; Lin, Chun-Yao; Tseng, Mei-Chen

Behavioral effects of titanium dioxide nanoparticles on larval zebrafish (Danio rerio)

2011

Chen, Te-Hao | Lin, Chun-Yao | Tseng, Mei-Chen

In this study, zebrafish embryos were exposed to titanium dioxide nanoparticles (TiO₂ NPs at 0.1, 0.5, 1, 5, 10mg/L or control) from fertilization to free swimming stage. Hatchability, survival, and malformation rate were not affected by TiO₂ NPs at these exposure levels. However, larval swimming parameters, including average and maximum velocity and activity level were significantly affected by TiO₂ NPs. Co-exposure to either the glutathione precursor, N-acetylcysteine (NAC), or the glutathione synthesis inhibitor, buthionine sulfoximine (BSO), did not significantly alter the behavioral effects resulting from TiO₂ NPs, suggesting that other factor(s) besides oxidative stress may contribute to the behavioral toxicity of TiO₂ NPs. Our study also demonstrated that the behavioral endpoints were more sensitive than the others (e.g., hatchability and survival) to detect toxicity of TiO₂ NPs on developing fish.

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