Functional characterization of a LAHC sucrose transporter isolated from grape berries in yeast
2008
Zhang, Ya Li | Meng, Qing Yong | Zhu, Hong Liang | Guo, Ying | Gao, Hong Yan | Luo, Yun Bo | Lu, Jiang
Large amounts of sugar are imported into grape berries from source leaves during ripening, and sucrose transporters play a key role during this process. In this study, a putative grape sucrose transporter gene VvSUC27, primarily expressed in sink tissue, was transformed into a yeast strain to characterize its function as a sucrose transporter. Sucrose was taken up by yeast transformed with VvSUC27 at an optimum pH of 4.0-5.0 and a K m of 8.0-10.5 mM, indicating VvSUC27 is a LAHC (low-affinity/high-capacity) sucrose transporter. The ability of sucrose uptake in transformed yeast was activated by monosaccharides and inhibited by maltose and DEPC.
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