Organosolv pretreatment of rice straw for efficient acetone, butanol, and ethanol production

Amiri, Hamid; Karimi, Keikhosro; Zilouei, Hamid

Organosolv pretreatment of rice straw for efficient acetone, butanol, and ethanol production

2014

Amiri, Hamid | Karimi, Keikhosro | Zilouei, Hamid

Acetone–butanol–ethanol (ABE) was produced from rice straw using a process containing ethanol organosolv pretreatment, enzymatic hydrolysis, and fermentation by Clostridium acetobutylicum bacterium. Pretreatment of the straw with 75% (v/v) aqueous ethanol containing 1% w/w sulfuric acid at 150°C for 60min resulted in the highest total sugar concentration of 31g/L in the enzymatic hydrolysis. However, the highest ABE concentration and productivity (10.5g/L and 0.20g/Lh, respectively) were obtained from the straw pretreated at 180°C for 30min. Enzymatic hydrolysis of the straw pretreated at 180°C for 30min with 5% solid loading resulted in glucose yield of 46.2%, which was then fermented to 80.3g butanol, 21.1g acetone, and 22.5g ethanol, the highest overall yield of ABE production. Thus, the organosolv pretreatment can be applied for efficient production of the solvents from rice straw.

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