Culture aspects of Isochrysis galbana for biodiesel production

Sanchez, Angel; Maceiras, Rocio; Cancela, Ángeles; Pérez, Alfonso

Culture aspects of Isochrysis galbana for biodiesel production

2013

Sanchez, Angel | Maceiras, Rocio | Cancela, Ángeles | Pérez, Alfonso

Biodiesel production from microalgae has recently increased on interest. The objective of this work is to explore the potential of biodiesel production from the microalgae Isochrysis galbana (T-ISO). For that reason, this microalgae was cultivated for biodiesel production. The culture system equipment consisted of two methacrylate ponds, 11mm thick. The pond has a base of 1.40×0.40m and a height of 0.4m. The influence of initial concentration of inoculum, stirring and carbon dioxide supply on the growth of algae were analyzed. The obtained results indicate that stirring improves the algae growth rate. The other two factors have no significant effect on final cell density. Obtained biomass was used to produce oil by extraction to use for biodiesel synthesis by means of basic-catalyzed reaction. For transesterification, microalgae oil was mixed with methanol (12:1 methanol to oil molar ratio), and sodium hydroxide (1%g NaOH/g oil) for 3h in a reactor at 62°C. The best harvest shows a biomass concentration of 0.305g/L with a FAME content of 12.5%.

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