Morphological and histopathological changes in seahorse (Hippocampus reidi) gills after exposure to the water-accommodated fraction of diesel oil

Delunardo, Frederico Augusto Cariello; Paulino, Marcelo Gustavo; Medeiros, Laila Carine Campos; Fernandes, Marisa Narciso; Scherer, Rodrigo; Chippari-Gomes, Adriana Regina

Morphological and histopathological changes in seahorse (Hippocampus reidi) gills after exposure to the water-accommodated fraction of diesel oil

2020

Industrial activities and urbanization are the main sources of pollutants in estuarine environments. Diesel, which is widely used in urban and port activities, is an important source of hydrocarbons in the aquatic environment, and its water-accommodated fraction (WAF) is toxic to the local biota. This study was performed to analyze the effects of diesel oil WAF on fish. Specifically, we characterized the gill morphology of the seahorse Hippocampus reidi and analyzed the histopathological changes in the gills after exposure to 50% diesel oil WAF. Acute (12, 24, 48, and 96 h) and subchronic (168 and 336 h) toxicity tests were performed. Furthermore, a recovery protocol was conducted: after exposure to 50% WAF for 168 h, the fish were transferred and kept in seawater without contaminants for 336 h, for a total experimental period of 504 h. The seahorse branchial apparatus was found to be tufted with short filaments; the apical surfaces of the pavement cells in the filament and lamellar epithelia formed “crests” and had microridges. Mitochondria-rich cells were distributed exclusively in the lamellar epithelium, while mucous cells were distributed in the filament epithelium. All pathologies observed after acute and subchronic exposure featured progressive time-dependent alterations of lamellar structure that might disrupt gill physiological and metabolic functions. During the recovery period, the gill alterations were gradually repaired.

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