Fungi in PAH-contaminated marine sediments: Cultivable diversity and tolerance capacity towards PAH

Álvarez-Barragán, Joyce; Cravo-Laureau, Cristiana; Wick, Lukas Y.; Duran, Robert

Fungi in PAH-contaminated marine sediments: Cultivable diversity and tolerance capacity towards PAH

2021

Álvarez-Barragán, Joyce | Cravo-Laureau, Cristiana | Wick, Lukas Y. | Duran, Robert

The cultivable fungal diversity from PAH-contaminated sediments was examined for the tolerance to polycyclic aromatic hydrocarbon (PAH). The 85 fungal strains, isolated in non-selective media, revealed a large diversity by ribosomal internal transcribed spacer (ITS) sequencing, even including possible new species. Most strains (64%) exhibited PAH-tolerance, indicating that sediments retain diverse cultivable PAH-tolerant fungi. The PAH-tolerance was linked neither to a specific taxon nor to the peroxidase genes (LiP, MnP and Lac). Examining the PAH-removal (degradation and/or sorption), Alternaria destruens F10.81 showed the best capacity with above 80% removal for phenanthrene, pyrene and fluoranthene, and around 65% for benzo[a]pyrene. A. destruens F10.81 internalized pyrene homogenously into the hyphae that contrasted with Fusarium pseudoygamai F5.76 in which PAH-vacuoles were observed but PAH removal was below 20%. Thus, our study paves the way for the exploitation of fungi in remediation strategies to mitigate the effect of PAH in coastal marine sediments.

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