The effect of Saccharomyces cerevisiae β-glucan on proliferation, phagocytosis and cytokine production of murine macrophages and dendritic cells

Javmen, Artur; Nemeikaitė-Čėnienė, Aušra; Grigiškis, Saulius; Lysovienė, Jelena; Jonauskienė, Irena; Šiaurys, Almantas; Mauricas, Mykolas

The effect of Saccharomyces cerevisiae β-glucan on proliferation, phagocytosis and cytokine production of murine macrophages and dendritic cells

2017

β-Glucan is a natural carbohydrate, which is produced by the variety of different organisms – bacteria, fungi, plants, etc. β-Glucan from different sources has been recognized as an active material, which is an immune stimulator for plants, invertebrates and mammals. Saccharomyces cerevisiae, the baker’s yeast, is one of the commonly used sources of β-1,3-glucan. The aim of the present work was to investigate how the different S. cerevisiaeβ-glucan preparations affect proliferation, phagocytosis and cytokine production of murine macrophages and dendritic cells. In our experiments, BALB/c macrophages and dendritic cells were treated with different β-glucan preparations in vitro. Then cell proliferation (AlamarBlue reagent), ability to phagocytose zymosan particles and production of IL-12 and IFNγ (Western blot) were investigated. Our results showed that β-glucan preparations stimulated proliferation of BALB/c macrophages and dendritic cells in vitro, but acted on phagocytosis and cytokine synthesis in different ways. This study demonstrated that S. cerevisiae β-glucan preparations propelled proliferation of the murine macrophages and dendritic cells and influenced phagocytosis and cytokine production of these cells. These effects can depend on the size of β-glucan molecules.

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