Effects of crude oil and oil/dispersant mixture on growth and expression of vitellogenin and heat shock protein 90 in blue crab, Callinectes sapidus, juveniles

Chiasson, Susan C.; Taylor, Caz M.

Effects of crude oil and oil/dispersant mixture on growth and expression of vitellogenin and heat shock protein 90 in blue crab, Callinectes sapidus, juveniles

2017

Chiasson, Susan C. | Taylor, Caz M.

The 2010 Deepwater Horizon (DWH) oil spill in the northern Gulf of Mexico (NGOM) resulted in over 780million liters of crude oil spilling into Gulf waters. In an effort to disperse the oil, nearly 7.6million liters of dispersant was applied. Many commercially and recreationally important species reside in or near the area of the spill. The blue crab, Callinectes sapidus, is common in the NGOM and is both economically and ecologically important in this region. In this study, after exposing juvenile blue crabs to oil or a mixture of oil and dispersant we tested for relative expression of heat shock protein 90 (hsp90) and vitellogenin (vtg) by measuring their corresponding mRNA expression. We also monitored crabs over two molts to test for effects on growth. Expression of hsp90 was significantly downregulated, and we did not detect any effects of exposure to oil or oil/dispersant mixture on growth or vtg expression.

Показать больше [+]