Negative regulation of P element excision by the somatic product and terminal sequences of P in Drosophila melanogaster

Handler, A.M.; Gomez, S.P.; O'Brochta, D.A.

Negative regulation of P element excision by the somatic product and terminal sequences of P in Drosophila melanogaster

1993

Handler, A.M. | Gomez, S.P. | O'Brochta, D.A.

A transient in vivo P element excision assay was used to test the regulatory properties of putative repressor-encoding plasmids in Drosophila melanogaster embryos. The somatic expression of an unmodified transposase transcription unit under the control of a heat shock gene promoter (phs pi) effectively repressed P excision in a dose-dependent manner at very low concentrations relative to somatically active transposase (encoded by the hs pi delta 2-3 gene). Maximum repression required transcription of the complete transposase gene, Dose-dependent repression of P excision was also observed in the presence of a vector plasmid (pCarnegie4) having only the terminal sequences, including transposase binding sites, of the P element. However, repression required considerably higher concentrations of pCarnegie4 than phs pi, and elimination of P excision was not observed.

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