Effects of combining Renlon with Renbarb1 and Renbarb2 on resistance of cotton (Gossypium hirsutum L.) to reniform nematode (Rotylenchulus reniformis Linford and Oliveria)

Gaudin, Amanda G.; Wallace, Ted P.; Scheffler, Jodi A.; Stetina, Salliana R.; Wubben, Martin J.

Effects of combining Renlon with Renbarb1 and Renbarb2 on resistance of cotton (Gossypium hirsutum L.) to reniform nematode (Rotylenchulus reniformis Linford and Oliveria)

2020

Gaudin, Amanda G. | Wallace, Ted P. | Scheffler, Jodi A. | Stetina, Salliana R. | Wubben, Martin J.

The upland cotton (Gossypium hirsutum L.) germplasm lines BARBREN-713, having the Renᵇᵃʳᵇ¹ and Renᵇᵃʳᵇ² quantitative trait loci (QTL), and LONREN, carrying the Renˡᵒⁿ QTL, have introgressed resistance to reniform nematode (Rotylenchulus reniformis Linford and Oliveria). Stunting has been observed in the LONREN source of resistance when subjected to heavy nematode pressure but not for BARBREN-713. To investigate the potential to reduce stunting in the LONREN source of resistance, cotton lines were developed with different combinations of reniform nematode resistance QTL from LONREN and BARBREN-713. Marker assisted selection was conducted to identify the following QTL combinations: (1) Renᵇᵃʳᵇ¹ + Renᵇᵃʳᵇ², (2) Renᵇᵃʳᵇ¹ + Renᵇᵃʳᵇ² + Renˡᵒⁿ, (3) Renᵇᵃʳᵇ² + Renˡᵒⁿ, (4) Renᵇᵃʳᵇ¹ + Renˡᵒⁿ, (5) Renᵇᵃʳᵇ¹ alone, (6) Renᵇᵃʳᵇ² alone, (7) Renˡᵒⁿ alone, and (8) a Null susceptible genotype. QTL combinations were inoculated with 0, 2300, or 5000 reniform nematodes per 120 cm³ soil in growth chamber tests. Plants were harvested at 60 days after inoculation, plant growth measurements made, and the nematode reproduction factor calculated. In this study, the Renᵇᵃʳᵇ¹ genotype was scored as susceptible to reniform nematode. Combining Renˡᵒⁿ with Renᵇᵃʳᵇ² did not improve resistance to reniform nematode; however, the Renᵇᵃʳᵇ²/Renˡᵒⁿ combination showed improved growth compared to LONREN-1, BARBREN-713, and the susceptible null check.

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