Development of an immunochromatographic assay for the detection of alternariol in cereal and fruit juice samples

Kong, Dezhao; Xie, Zhengjun; Liu, Liqiang; Song, Shanshan; Zheng, Qiankun; Guang, Hua

Development of an immunochromatographic assay for the detection of alternariol in cereal and fruit juice samples

2017

An immunochromatographic strip was developed for the determination of alternariol (AOH) using both semi-quantitative and quantitative methods based on a sensitive monoclonal antibody we produced. With AOH-spiked samples, the visual limit of detection for cereal and juice were 160 ng/g and 25 ng/mL, and the cut-off values were 640 ng/g and 100 ng/mL, respectively. For quantitative analysis based on a strip scan reader, the calculated limit of detection was 15.5–19.4 ng/g for cereal and 2.8–3.5 ng/mL for fruit juice. The recovery rates ranged from 116% to 125%, and 113% to 123%, respectively. Therefore, this method was effective for AOH detection and suitable for on-site detection and rapid screening of samples.

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