Effective rebaudioside a separation from stevia extracts by enzymatic bioconversion

Lee, Ha‐Nul; Hong, Seong‐Jin; Kang, HyeJin; Ju, Jung‐Hyun; Park, Bo‐Ram; Ko, Jin‐A; Kim, Young‐Min

Effective rebaudioside a separation from stevia extracts by enzymatic bioconversion

2021

Rebaudioside A with highly purity from commercialized stevia extracts is not amenable to preparative separation, owing to the structural similarity between stevioside and rebaudioside A, necessitating a new bioconversion method for its easy and simple separation. Complex stevia extracts were separated soluble or insoluble fractions after bioconversion with Penicillium decumbens‐derived β‐glucosidase (SPGase). Then, rebaudioside A or steviol was main component in the water soluble or insoluble. At 100 g/L commercialized stevia extracts scale, the enzymatic bioconversion gave the rebaudioside A (6.95 g/L) from soluble with highly purity and steviol (20.45 g/L) from insoluble. PRACTICAL APPLICATIONS: This one step‐enzymatic bioconversion with SPGase could be used for the separation of rebaudioside A from commercialized stevia extracts. This simple method is very useful application for low‐calorie sweetener in food materials.

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