Biosynthesis of pinene from glucose using metabolically-engineered Corynebacterium glutamicum

Kang, Min-Kyoung; Eom, Jin-Hee; Kim, Yunje; Um, Youngsoon; Woo, Han Min

Biosynthesis of pinene from glucose using metabolically-engineered Corynebacterium glutamicum

2014

Kang, Min-Kyoung | Eom, Jin-Hee | Kim, Yunje | Um, Youngsoon | Woo, Han Min

Pinene is a monoterpenes (C₁₀) that is produced in a genetically-engineered microbial host for its industrial applications in fragrances, flavoring agents, pharmaceuticals, and biofuels. Herein, we have metabolically-engineered Corynebacterium glutamicum, to produce pinene and studied its toxicity in C. glutamicum. Geranyl diphosphate synthases (GPPS) and pinene synthases (PS), obtained from Pinus taeda and Abies grandis, were co-expressed with over-expressed native 1-deoxy-d-xylulose-5-phosphate synthase (Dxs) and isopentenyl diphosphate isomerase (Idi) from C. glutamicum using CoryneBrick vector. Most strains expressing PS-GPPSs produced detectable amounts of pinene, but co-expression of DXS and IDI with PS (P. taeda) and GPPS (A. grandis) resulted in 27 μg ± 7 α-pinene g⁻¹ cell dry weight, which is the first report in C. glutamicum. Further engineering of PS and GPPS in the C. glutamicum strain may increase pinene production.

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