Purification and characterisation of high Mr glutenin subunit 20 and its linked y-type subunit from durum wheat

Buonocore, F.; Caporale, C.; Lafiandra, D.

Purification and characterisation of high Mr glutenin subunit 20 and its linked y-type subunit from durum wheat

1996

Buonocore, F. | Caporale, C. | Lafiandra, D.

High Mr glutenin subunit 20 and its linked y-type subunit, present in the durum wheat cultivar Lira, were purified by preparative reversed-phase high-performance liquid chromatography (RP-HPLC). Amino acid and N-terminal sequence analysis of subunit 20y confirmed that it corresponded to a y-type subunit. Moreover, the number and position of the cysteine residues in subunit 20 were determined by alkylation with the fluorogenic reagent 7-fluoro-4-sulfamoyl-2,1,3,-benzoxadiazole (ABD-F) and subsequent enzymic digestion with trypsin. N-terminal amino acid sequence analysis of the fluorescent peptides showed that subunit 20 had only two cysteine residues, one in the N-terminal region and the other in the C-terminal domain.

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