Tetracycline and sulfamethazine alter dissimilatory nitrate reduction processes and increase N2O release in rice fields

Shan, Jun; Yang, Pinpin; Rahman, M Mizanur; Shang, Xiaoxia; Yan, Xiaoyuan

Tetracycline and sulfamethazine alter dissimilatory nitrate reduction processes and increase N2O release in rice fields

2018

Shan, Jun | Yang, Pinpin | Rahman, M Mizanur | Shang, Xiaoxia | Yan, Xiaoyuan

Effects of antibiotics on the transformation of nitrate and the associated N₂O release in paddy fields are obscure. Using soil slurry experiments combined with ¹⁵N tracer techniques, the influence of tetracycline and sulfamethazine (applied alone and in combination) on the denitrification, anaerobic ammonium oxidation (anammox), dissimilatory nitrate reduction to ammonium (DNRA) and N₂O release rates in the paddy soil were investigated, while genes related to nitrate reduction and antibiotic resistance were quantified to explore the microbial mechanisms behind the antibiotics’ effects. The potential rates of denitrification, anammox, and DNRA were significantly (p < 0.05) reduced, which were mainly attributed to the inhibitory effects of the antibiotics on nitrate-reducing microbes. However, the N₂O release rates were significantly (p < 0.05) stimulated by the antibiotic treatments (0.6–6000 μg kg⁻¹ soil dry weight), which were caused by the different inhibition effects of antibiotics on N₂O production and N₂O reduction as suggest by the changes in abundance of nirS (nitrite reduction step) and nosZ (N₂O reduction to N₂ step) genes. Antibiotic resistance gene (tetA, tetG, sulI, and sulIII) abundances were significantly (p < 0.05) increased under high antibiotic exposure concentrations (>600 μg kg⁻¹ soil dry weight). Our results suggest that the widespread occurrence of antibiotics in paddy soils may pose significant eco-environmental risks (nitrate accumulation and greenhouse effects) by altering nitrate transformation processes.

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