Isolation of monoclonal antibodies that react preferentially with Listeria monocytogenes

Gavalchin, J.; Tortorello, M.L.; Malek, R.; Landers, M.; Batt, C.A.

Isolation of monoclonal antibodies that react preferentially with Listeria monocytogenes

1991

Gavalchin, J. | Tortorello, M.L. | Malek, R. | Landers, M. | Batt, C.A.

We have isolated and characterized three monoclonal antibodies that preferentially recognize the pathogen Listeria monocytogenes. Mouse monoclonal antibodies were produced by fusion of spleen cells from a Balb/c mouse, which was immunized with live L. monocytogenes, to an NS-1 myeloma partner. Hybridomas that produced antibodies which preferentially reacted with L. monocytogenes were identified by a direct binding enzyme-linked immunoabsorbent assay (ELISA) using a panel of bacteria which included L. ivanovii, Streptococcus faecalis, L. seeligeri, L. innocua and L. monocytogenes Scott A. Three hybridomas, Mab 20-10-2, Mab 36-6-12 and Mab 56-9-16 which preferentially reacted with L. monocytogenes were selected for further analysis. An indirect 'sandwich' ELISA was developed and used to further confirm that these hybridomas reacted with a number of L. monocytogenes isolates and not with other common cross-reacting bacteria including Kurthia sp.

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