Semi‐quantitative differences in gene transcription profiles between sexes of a marine snail by a new variant of cDNA‐AFLP analysis

MARTÍNEZ‐FERNÁNDEZ, M.; BERNATCHEZ, L.; ROLÁN‐ALVAREZ, E.; QUESADA, H.

Semi‐quantitative differences in gene transcription profiles between sexes of a marine snail by a new variant of cDNA‐AFLP analysis

2010

MARTÍNEZ‐FERNÁNDEZ, M. | BERNATCHEZ, L. | ROLÁN‐ALVAREZ, E. | QUESADA, H.

A variant of the cDNA‐AFLP method coupled to an automated sequencer was used to quantify transcripts differentially expressed between sexes of the marine snail Littorina saxatilis. First, we conducted a validation study of the technique using known concentrations of a commercial marker. Second, we analysed six replicates of males and females from a population showing no apparent sexual dimorphism. The results confirm that the method can be properly used within the range of DNA concentrations utilized. In addition, we detected a small percentage of spots (1.8%) differentially expressed between sexes, as expected from a low to moderately sexual dimorphic species.

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