Triarachidonoyl Glycerol Purification Process
2011
Venegas-Venegas, E. | Rincón-Cervera, M. A. | Guil-Guerrero, J. L.
Purification of arachidonic acid (AA, 20:4n-6) from different sources has been previously reported, but in most cases AA is obtained after triacylglycerol (TAG) hydrolysis. In this work, gravimetric normal-phase chromatography with gradient elution has been used to purify an AA-enriched fraction of TAG from the commercial single cell oil named ARASCO (38-44% AA content). A TAG fraction with more than 90% AA content was obtained, employing appropriate solvents for alimentary processing. The process was scaled up with satisfactory results. Due to the use of food-safe solvents in the whole process, it could be applied with alimentary or pharmaceutical purposes.
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