Restriction endonuclease Asi256I recognizes and cuts the nucleotide sequence 5′-GATC-3′

Ushakova, T. A.; Puchkova, L. I.; Gutorov, V. V.; Totmenina, O. D.; Repin, V. E.

Restriction endonuclease Asi256I recognizes and cuts the nucleotide sequence 5′-GATC-3′

2008

Ushakova, T. A. | Puchkova, L. I. | Gutorov, V. V. | Totmenina, O. D. | Repin, V. E.

A strain producing a restriction endonuclease was isolated from soil samples and identified as the Arthrobacter sp. strain Ck256. The enzyme produced by this strain was termed Asi256I. The isolation procedure for this enzyme was described, and the optimal conditions for its function were determined. It was shown that the restriction endonuclease Asi256I is a true isoschizomer of MboI, it has a temperature optimum of 6°C, and can be used in molecular-biological and genetic-engineering studies performed at low temperatures.

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