Environmentally realistic concentrations of the antibiotic Trimethoprim affect haemocyte parameters but not antioxidant enzyme activities in the clam Ruditapes philippinarum

Matozzo, Valerio; De Notaris, Chiara; Finos, Livio; Filippini, Raffaella; Piovan, Anna

Environmentally realistic concentrations of the antibiotic Trimethoprim affect haemocyte parameters but not antioxidant enzyme activities in the clam Ruditapes philippinarum

2015

Matozzo, Valerio | De Notaris, Chiara | Finos, Livio | Filippini, Raffaella | Piovan, Anna

Several biomarkers were measured to evaluate the effects of Trimethoprim (TMP; 300, 600 and 900 ng/L) in the clam Ruditapes philippinarum after exposure for 1, 3 and 7 days. The actual TMP concentrations were also measured in the experimental tanks. The total haemocyte count significantly increased in 7 day-exposed clams, whereas alterations in haemocyte volume were observed after 1 and 3 days of exposure. Haemocyte proliferation was increased significantly in animals exposed for 1 and 7 days, whereas haemocyte lysate lysozyme activity decreased significantly after 1 and 3 days. In addition, TMP significantly increased haemolymph lactate dehydrogenase activity after 3 and 7 days. Regarding antioxidant enzymes, only a significant time-dependent effect on CAT activity was recorded. This study demonstrated that environmentally realistic concentrations of TMP affect haemocyte parameters in clams, suggesting that haemocytes are a useful cellular model for the assessment of the impact of TMP on bivalves.

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