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Spectrophotometric method for differentiation of cardiac and hepatic lactate dehydrogenase activities in dogs

1989

Aguilera-Tejero, E. | Mayer-Valor, R. | Gomez-Cardenas, G.

To differentiate the origin of high total lactate dehydrogenase (LD) activity in canine sera, a spectrophotometric method based on the preferential inhibition of cardiac LD isoenzymes by pyruvate was performed. Comparison with the electrophoretic separation of LD isoenzyme activities and determination of the hydroxybutyrate dehydrogenase-to-LD ratio indicated that the method proposed gave a better discrimination between cardiac and hepatic LD activities than did the other tests.

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Ключевые слова АГРОВОК

animals blood diagnostic techniques dogs dogs enzyme activity enzyme inhibitors enzymology heart isoenzymes lactate dehydrogenase liver liver liver diseases metabolism methods myocardium necrosis pyruvic acid spectrophotometry spectroscopy

Библиографическая информация

Опубликовано в

American journal of veterinary research

Том 50 Выпуск 7 Нумерация страниц 1128 - 1130 ISSN 0002-9645

Другие темы

Dog diseases; Veterinary; Myocardial infarction; L-lactate dehydrogenase

Язык

Английский

Тип

Text; Journal Article

В АГРИСе с: 2024-02-28

Формат: MODS

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Эту запись предоставил National Agricultural Library

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