Spectrophotometric method for differentiation of cardiac and hepatic lactate dehydrogenase activities in dogs

Aguilera-Tejero, E.; Mayer-Valor, R.; Gomez-Cardenas, G.

Spectrophotometric method for differentiation of cardiac and hepatic lactate dehydrogenase activities in dogs

1989

Aguilera-Tejero, E. | Mayer-Valor, R. | Gomez-Cardenas, G.

To differentiate the origin of high total lactate dehydrogenase (LD) activity in canine sera, a spectrophotometric method based on the preferential inhibition of cardiac LD isoenzymes by pyruvate was performed. Comparison with the electrophoretic separation of LD isoenzyme activities and determination of the hydroxybutyrate dehydrogenase-to-LD ratio indicated that the method proposed gave a better discrimination between cardiac and hepatic LD activities than did the other tests.

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