Effects of dicyandiamide and acetylene on N2O emissions and ammonia oxidizers in a fluvo-aquic soil applied with urea

Wang, Qing; Zhang, Li-Mei; Shen, Ju-Pei; Du, Shuai; Han, Li-Li; He, Ji-Zheng

Effects of dicyandiamide and acetylene on N2O emissions and ammonia oxidizers in a fluvo-aquic soil applied with urea

2016

Ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) are crucial for N₂O emission as they carry out the key step of nitrification. Dicyandiamide (DCD) and acetylene (C₂H₂) are typical nitrification inhibitors (NIs), while the comparative effects of these NIs on N₂O production and ammonia oxidizers’ (AOB and AOA) growth are unclear. Four treatments including a control, urea, urea + DCD, and urea + C₂H₂ were set up to investigate their effect of inhibiting soil nitrification, nitrification-related N₂O emission as well as the growth of ammonia oxidizers with a fluvo-aquic soil using microcosms for 28 days. N₂O emission and net nitrification rate increased after the application of urea, but were significantly restrained in urea + NI treatments, while C₂H₂ was more effective in reducing N₂O emission and nitrification rate than DCD. The abundance of AOB, which was significantly correlated with N₂O emission and net nitrification rate, was more inhibited by C₂H₂ than DCD. Furthermore, the application of urea in all the soils had little impact on the AOA community, while obvious shifts of AOB community structure were found compared with the control. All AOB sequences fell within Nitrosospira cluster 3, and the AOA community was clustered to group 1.1b. Collectively, it indicated that application of urea combined with NIs (DCD or C₂H₂) could potentially alter N₂O emission, mainly through regulating the growth of AOB but not AOA in this fluvo-aquic soil.

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