Silica-based monolithic capillary columns—Effect of preparation temperature on separation efficiency

Planeta, Josef; Moravcová, Dana; Roth, Michal; Karásek, Pavel; Kahle, Vladislav

Silica-based monolithic capillary columns—Effect of preparation temperature on separation efficiency

2010

Planeta, Josef | Moravcová, Dana | Roth, Michal | Karásek, Pavel | Kahle, Vladislav

The temperature effects during the sol-gel process and ageing of the silica-based monolith on the structure and separation efficiency of the capillary columns (100μm i.d., 150mm) for HPLC separations were studied. The tested columns were synthesized from a mixture of tetramethoxysilane, polyethylene glycol and urea under the acidic conditions. The temperature was varied from 40°C to 44°C and formation of bypass channels between the silica mold and the capillary wall was examined. The temperature of 43°C was estimated as optimal for preparation of efficient silica capillary columns which were subsequently modified by octadecyldimethyl-N,N-diethylaminosilane or covered by poly(octadecyl methacrylate) and tested using standard mixture of alkylbenzenes under the isocratic conditions.

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