Molecular Identification of Diaspididae and Elucidation of Non-Native Species Using the Genes 28s and 16s
Campbell, Alexander M. | Lawrence, A. J. (Andrew J.) | Hudspath, Caleb B. | Gruwell, Matthew E.
Armored scale insects pose a serious threat to habitat conservation across the globe because they include some of the most potent invasive species in the world. They are such a serious concern because their basic morphology, small size, and polyphagous feeding habits often allow them to exist undetected by growers and quarantine experts. In order to provide a potential solution to the problem, we have attempted to elucidate the effectiveness of molecular identification techniques using ribosomal 28s and endosymbiotic 16s rRNA. Sequence data was obtained from many field-collected insects to test the feasibility of identification techniques. A protocol for quick species determination based on sequence data is provided.
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