X-ray Radiation Induces Deprotonation of the Bilin Chromophore in Crystalline D. radiodurans Phytochrome

Li, Feifei; Burgie, E Sethe; Yu, Tao; Héroux, Annie; Schatz, George C.; Vierstra, Richard D.; Orville, Allen M.

X-ray Radiation Induces Deprotonation of the Bilin Chromophore in Crystalline D. radiodurans Phytochrome

2015

We report that in the red light-absorbing (Pr) state, the bilin chromophore of the Deinococcus radiodurans proteobacterial phytochrome (DrBphP) is hypersensitive to X-ray photons used in typical synchrotron X-ray protein crystallography experiments. This causes the otherwise fully protonated chromophore to deprotonate without additional major structural changes. These results have major implications for our understanding of the structural and chemical characteristics of the resting and intermediate states of phytochromes and other photoreceptor proteins.

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Ключевые слова АГРОВОК

photoreceptors phytochrome

Библиографическая информация

Опубликовано в

Journal of the American Chemical Society

Том 137 Выпуск 8 Нумерация страниц 2792 - 2795 ISSN 1520-5126

Издатель

American Chemical Society

Другие темы

X-radiation; Crystallography; Deinococcus radiodurans; Photons

Язык

Английский

Лицензия

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Тип

Journal Article; Text

В АГРИСе с: 2024-02-29

Формат: MODS

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Эту запись предоставил National Agricultural Library

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Ссылки

DOI http://dx.doi.org/10.1021%2Fja510923m

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