1,3-Dioleoyl-2-palmitoylglycerol-rich triacylglycerol characterization by three processing methods
Wang, Zhiyu | Liu, Lihua | Liu, Libo | Liu, Tingting | Li, Chun | Sun, Lihua
1,3-Dioleoyl-2-palmitoylglycerol (OPO) is a kind of structured triglyceride which could instead human milk fat (HMF) for the fat of infant formula, this kind of structured triglyceride is good at absorbing easily. The aim of this paper was to produce OPO though three different processing technologies. The raw material for the first method (Method 1) and second method (Method 2) was palm oil, and their catalyst is Novozym 435 and Lipozyme TL IM. The difference was the Method 1, which obtained tripalmitin (PPP) by acidolysis palm oil and palmitic acid (PA), then obtained OPO by acidolysis PPP and oleic acid (OA), Method 2 was acidolysis palm oil and mixed fatty acids directly without crystallization. The third method (Method 3) produced palm stearin (PS) enriched in PA by catalyzing palm stearin with sodium methoxide firstly, and then palm stearin enriched in PA and mixed vegetable oils catalyzed by Lipozyme TL IM. The content of OPO and sn-2 position PA of three methods was higher than 40.34% and 39.34%. The relative content of PA located at the sn-2 position and OA located at the sn-1, 3 position was higher than 59.97% and 66.01%. Among the three methods, the methods related to enzyme appeared safer, and Method 3 was easier for manufacturing OPO and saved time, so it should be more suitable for industrial production.
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