Dose–response analysis of potassium bromate–induced toxicity in Allium cepa L. meristematic cells

Öztürk, Gökçe; Çavuşoğlu, Kültiğin; Yalçın, Emine

Dose–response analysis of potassium bromate–induced toxicity in Allium cepa L. meristematic cells

2020

Öztürk, Gökçe | Çavuşoğlu, Kültiğin | Yalçın, Emine

In this study, the toxic effects of potassium bromate (KBrO₃) were tested on Allium cepa L. meristematic cells. In order to determine the toxic effect and dose relationship, KBrO₃ toxicity was investigated at doses of 25, 50, and 100 mg/L. The toxic effects were evaluated by using cytogenetic, biochemical, anatomical, and physiological parameters, and serious damages were observed depending on the dose. Significant reductions in germination percentage, weight gain, and radicle length were observed in all KBrO₃-treated groups compared with the control. Mitotic activity decreased in meristematic cells after KBrO₃ application. and mitotic index was decreased by 1.8 times in 100 mg/L KBrO₃-treated group compared with the control group. The frequencies of micronucleus and chromosomal abnormalities tested as cytogenetic parameters were significantly higher in the group treated with 100 mg/L KBrO₃ than those in the control group. Fragment and sticky chromosome were the most common types of chromosomal abnormalities. Lipid peroxidation measured in terms of MDA content increased with increasing doses of KBrO₃. The activities of catalase and superoxide dismutase as antioxidant enzymes were importantly changed in KBrO₃-treated groups. Anatomical changes such as cell deformation, substance accumulation, cell wall thickening, and flattened nucleus were determined after KBrO₃ application, and it was observed that these changes reached a maximum level at 100 mg/L dose of KBrO₃. As a result, KBrO₃ treatments were been found to cause physiological, biochemical, cytogenetic, and anatomically toxic effects in meristematic cells of A. cepa, a eukaryotic model organism. The versatile toxicity induced by KBrO₃ increased depending on the dose and reached a maximum level at 100 mg/L.

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