Rutin directly affects stimulatory action of FSH on the ovarian cell

Sirotkin, Alexander V.; Pelleova, Betina; Fabova, Zuzana; Makovicky, Pavol; Alwasel, Saleh; Harrath, Abdel Halim

Rutin directly affects stimulatory action of FSH on the ovarian cell

2021

The influence of plant isoflavone rutin (vitamin P) on healthy ovarian cells is unknown.We evaluated the effect of FSH alone, at various dose levels (0, 10, 100, or 1000 ng/mL), and in combination with rutin (10 μg/mL) on the markers of viability (trypan blue test), proliferation (expression of proliferating cell nuclear antigen, PCNA and cyclin B1), apoptosis (expression of Bcl-2-associated X protein, bax and caspase 3) (by quantitative immunocytochemistry), and release of progesterone (P), testosterone (T), and estradiol (E) (by ELISA) in cultured porcine ovarian granulosa cells.FSH increased cell viability, proliferation, and release of steroid hormones; FSH inhibited apoptosis. When added alone, rutin decreased cell viability, proliferation and apoptosis and promoted P, T and E output. Furthermore, when added together with FSH, it suppressed all the FSH effects.The present observations demonstrate the ability of rutin to directly promote ovarian cell steroidogenesis and to suppress their viability, proliferation, apoptosis, and response to the stimulatory action of FSH. Such adverse effects of rutin on ovarian cells should be taken into account regarding consumption of food and use of medical preparations containing rutin and its metabolites.

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