Expression of a Brassica napus malate synthase gene in transgenic tomato plants during the transition from late embryogeny to germination

Comai, L.; Matsudaira, K.L.; Heupel, R.C.; Dietrich, R.A.; Harada, J.J.

Expression of a Brassica napus malate synthase gene in transgenic tomato plants during the transition from late embryogeny to germination

1992

Comai, L. | Matsudaira, K.L. | Heupel, R.C. | Dietrich, R.A. | Harada, J.J.

To study gene regulation during the transition from late embryogeny to germination, we have analyzed the expression of a gene encoding the glyoxylate cycle enzyme malate synthase in transgenic tomato (Lycopersicon esculentum) plants. We have shown that although there are at least four classes of malate synthase genes in Brassica napus L., one gene is expressed at a high level during both late embryogeny and postgermination. Analyses of transgenic tomato plants containing the expressed B. napus gene along with 4.7 and 1.0 kilobase pairs of 5' and 3' flanking sequences, respectively, confirmed that a single gene is expressed at both stages of development. Furthermore, localization studies have shown that mRNA encoded by the B. napus gene is distributed throughout the tissues of a mature embryo but is not detected in the vascular cylinder of a seedling. We conclude that the sequences required to qualitatively regulate the gene correctly over the plant life cycle are present within the transferred gene and/or flanking regions. Moreover, the malate synthase gene is regulated differently during late embryogeny and postgermination in the developing vascular cylinder.

Показать больше [+]