Comparative clustering and genotyping of Campylobacter jejuni strains isolated from broiler and turkey feces by using RAPD-PCR and ERIC-PCR analysis

Staji, Hamid; Birgani, Soghra Farhani; Raeisian, Behnaz

Comparative clustering and genotyping of Campylobacter jejuni strains isolated from broiler and turkey feces by using RAPD-PCR and ERIC-PCR analysis

2018

Staji, Hamid | Birgani, Soghra Farhani | Raeisian, Behnaz

To compare the genetic profiles of Campylobacter jejuni (C. jejuni) isolates of broiler and turkey reservoirs sampled in Semnan City, Iran, 60 C. jejuni isolates (30 from broilers and 30 from turkeys) were genotyped by RAPD-PCR- and ERIC-PCR-based methods. RAPD-PCR identified 6 genotypes and ERIC-PCR identified 21 genotypes among the 60 C. jejuni isolates. Both techniques were able to discriminate the C. jejuni isolates. Results demonstrated that one single genotype was identical to broiler and one single genotype was identical to turkey isolates at 83% similarity level in RAPD UPGMA clustering. Also, one single profile was identical to turkey isolates at 73% similarity level in ERIC-PCR clustering. The existence of high genetic similarity in some C. jejuni isolates from both hosts suggests the presence of some overlap between isolates from different sources and boosts the power of RAPD-PCR- and ERIC-PCR-based methods in discriminating C. jejuni isolates from various sources.

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