In vitro effect of T-2 mycotoxin on the immune response of mice

Holt, P.S.; DeLoach, J.R.

In vitro effect of T-2 mycotoxin on the immune response of mice

1988

Holt, P.S. | DeLoach, J.R.

The in vitro biologic effects of T-2 mycotoxin on the immune response of mice was undertaken. Twenty nanograms of toxin abrogated the immune response to the T-dependent antigen sheep RBC, whereas a partial response was observed when 2 ng was used. Analysis of cell culture viabilities indicated that cell death occurred with toxin doses that conincided with the diminished immune responses. A similar decreased response was observed against the T-independent antigen, TNP-lipopolysaccharide, indicating toxic effects on both B and T lymphocyte populations. Delay of toxin administration as much as 116 hours of the 120-hour incubation period still resulted in a substantially diminished immune response, indicating the toxin acts on both the afferent and efferent immune systems. Equal effects were observed for mice of the b, d, and k haplotype, indicating no apparent strain variability in sensitivity to T-2 mycotoxin effects. These results indicated that T-2 mycotoxin can modulate the immune response, and that this modulation is attributable to direct toxic effects on the cells of the immune system.

Показать больше [+]