Effects of Unripe Pear Extract on UVB-Irradiated Skin Wrinkles

Kim, D.K.; Lee, M.H.; Park, G.D.; Kim, S.R.; Ham, W.J.; Lee, J.M.

Effects of Unripe Pear Extract on UVB-Irradiated Skin Wrinkles

2023

Acute and chronic ultraviolet (UV) irradiation causes inhibition of collagen synthesis, over-expression of matrix melalloproteinase (MMPs), and activation of apoptosis, leading to skin photo-aging. In this study, we investigated the protective effects of unripe pear extract (UPhenon®, UP) against ultraviolet B (UVB)-irradiated skin damage in human skin fibroblasts cell (HS27 cell). To identify the protective effects of UP against UVB irradation, transforming growth factor beta receptor 1 (TGFR1), procollagen C-endopeptidase enhancer (PCOLCE), and type Ⅰ collagen were measured by real-time polymerase chain reaction (PCR) and the apoptosis related factors (c-Jun N-terminal kinase [JNK], c-Fos, and c-Jun), MMPs (1, 3, 9) and mothers against decapentaplegic homolog 3 (Smad3) were determined by western blot. UP significantly increased the following: mRNA expression and protein activation of TGFR1, PCOLCE, type Ⅰ collagen, and Smad3. However, UP significantly decreased the protein activation of apoptosis-related factors and MMPs. Our findings suggest that UP has a protection effects against UVB-irradiated skin photoaging in human skin fibroblasts through inhibition of apoptosis and MMPs and the activation of collagen synthesis. Thus, UP is believed to have the potential to be develop as a funtional natural food for improveing skin health.

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