Analysis of Licorice Extract Components to Enhance Commercial Use and Increase Extraction Yields
2023
Lee, E.H. | Jeong, H.M. | Kim, E.A. | Lee, Y.R. | Shim, J.H.
Licorice (Glycyrrhiza uralensis) has various physiological activities and has been used to treat various diseases, including respiratory, inflammatory, and skin diseases. These therapeutic effects have been attributed to flavonoids and saponins. Glycyrrhizin is the most abundant saponin in licorice, and liquiritin (a flavonoid) is a representative compound found in licorice known for its antiviral and antioxidant properties. In this study, an extraction method was developed to facilitate the domestic and commercial use of glycyrrhizin and liquiritin, and an HPLC program was optimized to conduct component analysis. Following extraction with 40% (v/v) ethanol at 40°C for 60 minutes, 3.4 mg/g of glycyrrhizin and 0.7 mg/g of liquiritin were obtained from licorice extract. In contrast to existing methods that focus on the analysis of a broad spectrum of constituents, the devised extraction method places emphasis on the analysis of components intended for commercial use in licorice and is based on the optimization of the extraction of glycyrrhizin and liquiritin, the primary active compounds in licorice. Our method takes into account extraction time, temperature, ethanol concentration, and solvent ratio, which makes it suitable for analyzing and adjusting extraction yields based on specific requirements.
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