EXPLORING GENETIC DIVERSITY IN AZERBAIJANI BARLEY COLLECTIONTHROUGH AMPLICON SEQUENCING

Mehraj Abbasov,; Sevda Babayeva; Khanbala Rustamov; Laman Rasulova; Ofelya Rahi��mova; Afat Mammadova; Vusala Izzatullayeva; Elchi��n Haji��yev; Rami��z Ali��yev,zeynal Akparov

EXPLORING GENETIC DIVERSITY IN AZERBAIJANI BARLEY COLLECTIONTHROUGH AMPLICON SEQUENCING

2023

In this reasearch, genotyping was conducted on 86 accessions of wild barley (Hordeum vulgare L. subsp. spontaneum C.Koch) and 85 accessions of cultivated barley (Hordeum vulgare L. subsp. vulgare) collected from 20 different regions in Azerbaijan. Amplicon sequencing technology was employed, revealing 255 specific single-nucleotide polymorphism (SNP) markers of a polymorphic nature. Out of the total options, 66% were transitions, and 34% were transversions. For the studied barley collection, the Genetic Diversity Index (GDI) and Polymorphism Information Content (PIC) were calculated as 0.347 and 0.280, respectively. Notably, the 6H chromosome exhibited the highest number of SNP markers, whereas the 7H chromosome displayed the fewest. Utilizing cluster analysis, the accessions were categorized into three main groups. Remarkably, the study successfully differentiated between genotypes of wild and cultivated barley. Employing the STRUCTURE program, the number of subpopulations (K) ranged from 2 to 5. The model with K = 4 was found to be the most consistent with the collection’s structure. The SNP markers acquired through multiplex amplicon sequencing demonstrate robust utility for diverse assessments and analyses within various collections. They prove suitable for exploring genetic variability and relationships, both in barley overall and in H. vulgare and H. spontaneum individually.

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