Regeneration of Plant according to Somatic Embryogenesis Types in Cnidium officinale Makino
2022
Kim, J.A. | Jeong, H.Y.
BACKGROUND: The present study investigated the types and concentrations of plant hormones affecting somatic embryo induction from leaves, stems, and roots of Cnidium officinale Makino. METHODS AND RESULTS: Somatic embryos could be induced using two methods: direct and indirect somatic embryogenesis. Direct somatic embryogenesis was induced when the whole explants were grown in a medium supplemented with cytokinin (e.g., kinetin), while indirect somatic embryogenesis was induced when the medium was supplement with auxin [eg., 2,4-dichlorophenoxyacetic acid (2,4-D)]. Both direct and indirect somatic embryos were mostly induced from leaves. The highest direct somatic embryo induction (95.8%) was obtained from leaf explants grwon on Murashige and Skoog (MS) medium supplemented with 0.5 - 1.0 mg/ℓ kinetin. The highest indirect somatic embryo induction (75.0%) was obtained from leaf explants grown on MS medium supplemented with 0.5 mg/ℓ 2,4-D. The induced somatic embryo could successfully germinate and acclimatize. CONCLUSIONS: Our results can be useful for establising a mass propagation protocol for C. officinale.
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