Molecular detection of blaTEM gene for encoding extended spectrum beta-lactamase (ESBL) on Escherichia coli isolated from deer feces in Indonesia

Katty Hendriana Priscilia Riwu; Mustofa Helmi Effendi; Fedik Abdul Rantam; Aswin Rafif Khairullah; Shendy Canadya  Kurniawan; Ahmad Kurniawan; Ikechukwu Benjamin  Moses; Abdullah Hasib; Agus Widodo; Sheila Marty  Yanestria; Otto Sahat Martua  Silaen; Amir Shehzad

Molecular detection of blaTEM gene for encoding extended spectrum beta-lactamase (ESBL) on Escherichia coli isolated from deer feces in Indonesia

2024

Antibiotic resistance is a serious global health threat and broad-spectrum beta-lactamase- producing Enterobactericeae (ESBL) are major contributors. This research focuses on the presence of Eschericia coli as a producer of ESBL in the fresh feces of deer. This study aims to gain deeper insight into ESBL sourced from deer. 129 fresh stool samples were collected. A total of 89 samples came from the East Java region and 40 samples came from the East Nusa Tenggara region. Identification were carried out which was then followed by PCR. We found 9 positive samples of ESBL-producing E. coli containing the blaTEM gene as the ESBL encoding gene. 33 samples (25.28%) were positive for E. coli. 8 isolates (24.24%) were multidrug-resistant, East Java (6 isolates) and East Nusa Tenggara (2 isolates). All isolates of multidrug-resistant E. coli 7 isolates were ESBL positive (87.5%) and also contained the blaTEM gene. The presence of MDR and ESBL-producing E. coli isolates have been confirmed in deer. Deer should be considered as a source of MDR and ESBL transmission for public health. The conclusion of this study is to reveal the presence of ESBL-producing E. coli originating from deer. The importance of this cross-sector collaboration to monitor antimicrobial resistance.

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