Phylogenetic study based on the phosphoprotein gene of Iranian Newcastle disease viruses (NDV) isolates, 2010 -2012
2014
Ghalyanchi Langeroudi, Arash | Hossein, Hossein | Karimi, Vahid | Madadgar, Omid | Hashemzadeh, Masoud | Ghafouri, Seyed Ali | Bagheri, Seyed Sina | Vahedi, Seyed Milad
BACKGROUND: Newcastle disease virus (NDV) is the causativeagent of the Newcastle disease (ND), a highly contagiousdisease in birds that causes significant economic losses to thepoultry industry worldwide. ND is endemic in Iran and outbreaksare reported regularly in commercial poultry flocks and differentspecies of birds. OBJECTIVES:The current study was carried outto characterize NDV based on phosphorprotein (P) gene fromrecent outbreaks in Iran, 2010-2012. METHODS: The P genefragment of NDV isolates of five chickens, 1 ostrich, and 1Pigeon paramyxovirus-1 was obtained by RT-PCR and sequenced.RESULTS: Phylogenetic analysis of sequences revealed thatchicken and ostrich NDV isolates were closely related andplaced in the genotype VII and Pigeon Paramyxovirus-1 waslocated in the genotype V. CONCLUSIONS:This is the first reportof Phosphoprotein gene sequences of NDV strains isolated inIran. This study will help us to understand the epidemiology andmolecular characteristics of Newcastle disease virus in Iran.
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