Establishment of Spinal Cord Injury Model in Nonhuman Primate (Rhesus Macaca Mulatta) with Royan Impactore Device
2021
Hajinasrollah, Mostafa | Sharifi, Davood | Kiani, Sahar | Soroori, Sarang | Jabbari, Reza | Mirsadeghi, Ehsan | Mokhtari, Roshanak
BACKGROUND: There have been many reports on spinal cord injuries in humans and animals, which is a major medical and veterinary concern; therefore, the present research project was carried out to devise an appropriate treatment approach. OBJECTIVES: The purpose of the present study was to apply the modified Allen’s test and Royan Impactor Devicein order to 1) induce spinal cord injury on rhesus monkeys and 2) gain the ultimate confirmation by clinical examinations and MR imaging for application in human and veterinary medicine. METHODS: Royan Impactor Device was used to induce spinal cord injury on rhesus monkeys. Five rhesus monkeys were anesthetized with ketamine, xylazine, and isoflurane. Laminectomy was done at the level of T9-T10, and a 50 g weight was then dropped from a 12 cm height through a guide tube onto a 10 mm2 impact plate on the exposed spinal cord. RESULTS: The results of the present study showed signal changes with decreasing the trend in the T2W of spinal cord in sagittal and axial areas due to hematoma, inflammation, pressure on spinal cord, and tissue destruction after spinal cord injury. In the clinical examination of all samples, spinal cord shock symptoms, marked by bladder distention, disappeared 3-5 days post spinal cord injury. Not all measurable factors related to the severity of the injury were restored to the normal condition until the end of the study although their severities were reduced to some extend. CONCLUSIONS: Modified Allen’s test and the instrument that we designed for this study were able to satisfy the needs of the researchers in treating the spinal cord injury.
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