Optimization and Modeling of Curdlan Production under Multi-physiological-parameters Process Control by Agrobacterium radiobacter Mutant A-15 at High Initial Glucose
Yang, H. | Li, W.Q. | Chen, S.C. | Guo, X.L. | Huang, F. | Zhu, P.
The optimization and kinetic modeling of curdlan synthesis by Agrobacterium radiobacter mutant A15 under multi-physiological-parameters process control, especially employing the on-line oxygen uptake rate (OUR) and carbon dioxide evolution rate (CER) were performed in a 50-L fermentor. Results indicated that the maximum production of curdlan reach about 32.5 g/L with a glucose conversion yield of 49.5%, the maximum specific growth rate was about 0.5338 h⁻¹, the kinetics equation were of positive significance for production. It also demonstrated that A-15 was insensitive to catabolite repression during polysaccharide production for its maintaining high conversion yield in the medium contained 6.6% (W/V) initial glucose. In addition, the OUR and CER at the stationary growth stage showed a positive correlation with curdlan synthesis. This work will provide more theoretical support for large-scale curdlan production.
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