Crossover rate between homologous chromosomes and interference are regulated by the addition of specific unpaired chromosomes in Brassica
2014
Suay Llopis, Maria Loreto | Zhang, Deshuang | Eber, Frederique | Jouy, Helene | Lodé-Taburel, Maryse | Huteau, Virginie | Coriton, Olivier | Szadkowski, Emmanuel | Leflon, Martine | Martin, Olivier | Falque, Matthieu | Jenczewski, Eric | Paillard, Sophie | Chèvre, Anne-Marie | Institut de Génétique, Environnement et Protection des Plantes (IGEPP) ; Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST | Beijing Vegetable Research Center (BVRC) of BAAFS ; National Engineering Research Center for Vegetables (NERCV) | Terres Inovia | Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) (GQE-Le Moulon) ; Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS) | Institut Jean-Pierre Bourgin (IJPB) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech | Foundation Louis D. - Institut de France; Plant Breeding Departement - INRA; 'ANR - Agence Nationale de la Recherche - The French National Research Agency' [BLANC07-3_188863188863]; Plant Breeding Department - INRA
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Показать больше [+] Меньше [-]Английский. Recombination is a major mechanism generating genetic diversity, but the control of the crossover rate remains a key question. In Brassica napus (AACC, 2n=38), we can increase the homologous recombination between A genomes in AAC hybrids. Hypotheses for this effect include the number of C univalent chromosomes, the ratio between univalents and bivalents and, finally, which of the chromosomes are univalents. To test these hypotheses, we produced AA hybrids with zero, one, three, six or nine additional C chromosomes and four different hybrids carrying 2n=32 and 2n=35 chromosomes. The genetic map lengths for each hybrid were established to compare their recombination rates. The rates were 1.4 and 2.7 times higher in the hybrids having C-6 or C-9 alone than in the control (0C). This enhancement reached 3.1 and 4.1 times in hybrids carrying six and nine C chromosomes, and it was also higher for each pair of hybrids carrying 2n=32 or 2n=35 chromosomes, with a dependence on which chromosomes remained as univalents. We have shown, for the first time, that the presence of one chromosome, C-9, affects significantly the recombination rate and reduces crossover interference. This result will have fundamental implications on the regulation of crossover frequency.
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