First report and comparative genomics analysis of a blaoxa-244-haarboring escherichia coli isolate recovered in the American continent | First report and comparative genomics analysis of a blaoxa-244-haarboring escherichia coli isolate recovered in the American continent
2019
Abril Riaño, Deisy Julieth | Bustos Moya, Ingrid Gisell | Márquez-Ortiz, Ricaurte Alejandro | Josa, Diego | Corredor Rozo, Zayda Lorena | Torres Molina, Isabel | Vanegas Gómez, Natasha | Escobar-Pérez, Javier | Escobar-Pérez, Javier [0000-0002-0432-6978] | Abril Riaño, Deisy Julieth [0000-0002-6686-6283]
The carbapenemase OXA-244 is a derivate of OXA-48, and its detection is very difficult in laboratories. Here, we report the identification and genomic analysis of an Escherichia coli isolate (28Eco12) harboring the blaOXA-244 gene identified in Colombia, South America. The 28Eco12 isolate was identified during a retrospective study, and it was recovered from a patient treated in Colombia. The complete nucleotide sequence was established using the PacBio platform. A comparative genomics analysis with other blaOXA-244–harboring Escherichia coli strains was performed. The 28Eco12 isolate belonged to sequence type (ST) 38, and its genome was composed of two molecules, a chromosome of 5,343,367 bp and a plasmid of 92,027 bp, which belonged to the incompatibility group IncY and did not harbor resistance genes. The blaOXA-244 gene was chromosomally encoded and mobilized by an ISR1-related Tn6237 composite transposon. Notably, this transposon was inserted and located within a new genomic island. To our knowledge, this is the first report of a blaOXA-244–harboring Escherichia coli isolate in America. Our results suggest that the introduction of the OXA-244-producing E. coli isolate was through clonal expansion of the ST38 pandemic clone. Other isolates producing OXA-244 could be circulating silently in America.
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