Pseudomonas aeruginosa host-pathogen interactions in human corneal infection models
2020
Cendra Gascón, María del Mar | Elsahn, Ahmad | Humbert, Maria Victoria | Christodoulides, Myron | Dua, Harminder | Hossain, Parwez | Universitat Politècnica de Catalunya. Departament d'Enginyeria Agroalimentària i Biotecnologia
In the whole tissue model (WTM), donor buttons were infected with PAO1 by scoring and intrastromal in-jection. Tissue was examined after 3, 9 and 24 h post challenge by transmission electron microscopy (TEM) andscanning electron microscopy (SEM).In the cell culture model (CCM), cornealfibroblasts (CF) were infected in vitro with PAO1. Bacterial ad-herence and internalization were assayed at 3, 6 and 9 h by SEM and TEM. Adherent and internalized bacteriawere measured by the gentamicin protection invasion assay
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