The anti-influenza M2e antibody response is promoted by XCR1 targeting in pig skin
2017
Deloizy, Charlotte | Fossum, Even | Barnier-Quer, Christophe | Urien, Celine | Chrun, Tiphany | Duval, Audrey | Codjovi, Maëlle | Bouguyon, Edwige | Maisonnasse, Pauline | Herve, Pierre Louis | Barc, Céline | Boulesteix, Olivier | Pezant, Jérémy | Chevalier, Christophe | Collin, Nicolas | Dalod, Marc | Bogen, Bjarne | Bertho, Nicolas | Schwartz-Cornil, Isabelle | Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)) ; Institut National de la Recherche Agronomique (INRA) | Université Paris Saclay (COmUE) | University of Oslo (UiO) | Université de Lausanne = University of Lausanne (UNIL) | Plateforme d'Infectiologie Expérimentale (PFIE) ; Institut National de la Recherche Agronomique (INRA) | Aix Marseille Université (AMU) | Agence National de la Recherche grant DCskin-VacFlu [ANR 2011-ISV3-001-01]; Research Council of Norway [220642/H10]; K.G. Jebsen Foundation | ANR-11-ISV3-0001,DCskinVacFlu,Développement de vaccins de pointe par ciblage des cellules dendritiques de la peau dans le modèle pré-clinique porcin (exemple de la grippe)(2011)
International audience
Показать больше [+] Меньше [-]Английский. XCR1 is selectively expressed on a conventional dendritic cell subset, the cDC1 subset, through phylogenetically distant species. The outcome of antigen-targeting to XCR1 may therefore be similar across species, permitting the translation of results from experimental models to human and veterinary applications. Here we evaluated in pigs the immunogenicity of bivalent protein structures made of XCL1 fused to the external portion of the influenza virus M2 proton pump, which is conserved through strains and a candidate for universal influenza vaccines. Pigs represent a relevant target of such universal vaccines as pigs can be infected by swine, human and avian strains. We found that cDC1 were the only cell type labeled by XCR1-targeted mCherry upon intradermal injection in pig skin. XCR1-targeted M2e induced higher IgG responses in seronegative and seropositive pigs as compared to non-targeted M2e. The IgG response was less significantly enhanced by CpG than by XCR1 targeting, and CpG did not further increase the response elicited by XCR1 targeting. Monophosphoryl lipid A with neutral liposomes did not have significant effect. Thus altogether M2e-targeting to XCR1 shows promises for a trans-species universal influenza vaccine strategy, possibly avoiding the use of classical adjuvants.
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