<i>ct2vl</i>: A Robust Public Resource for Converting SARS-CoV-2 Ct Values to Viral Loads
2024
Elliot D. Hill | Fazilet Yilmaz | Cody Callahan | Alex Morgan | Annie Cheng | Jasper Braun | Ramy Arnaout
The amount of SARS-CoV-2 in a sample is often measured using Ct values. However, the same Ct value may correspond to different viral loads on different platforms and assays, making them difficult to compare from study to study. To address this problem, we developed <i>ct2vl</i>, a Python package that converts Ct values to viral loads for any RT-qPCR assay/platform. The method is novel in that it is based on determining the maximum PCR replication efficiency, as opposed to fitting a sigmoid (S-shaped) curve relating signal to cycle number. We calibrated <i>ct2vl</i> on two FDA-approved platforms and validated its performance using reference-standard material, including sensitivity analysis. We found that <i>ct2vl</i>-predicted viral loads were highly accurate across five orders of magnitude, with 1.6-fold median error (for comparison, viral loads in clinical samples vary over 10 orders of magnitude). The package has 100% test coverage. We describe installation and usage both from the Unix command-line and from interactive Python environments. <i>ct2vl</i> is freely available via the Python Package Index (PyPI). It facilitates conversion of Ct values to viral loads for clinical investigators, basic researchers, and test developers for any RT-qPCR platform. It thus facilitates comparison among the many quantitative studies of SARS-CoV-2 by helping render observations in a natural, universal unit of measure.
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