Rapid detection of clonidine and its cross-reactivity with apraclonidine in pig urine using an immunochromatographic test strip

Min Feng; Steven Suryoprabowo; Hong Tao; Liqiang Liu; Qiankun Zheng; Hua Kuang

Rapid detection of clonidine and its cross-reactivity with apraclonidine in pig urine using an immunochromatographic test strip

2018

Clonidine (CLO) preferentially stimulates central α-2-adrenoceptors and causes both desirable and undesirable effects. Undesirable effects include sedation and withdrawal reactions, which occur as a sudden rise in arterial pressure, nervousness, agitation, and increased heart rate. An immunochromatographic test strip was developed for the rapid simultaneous detection of CLO and its cross-reactivity with apraclonidine (ACLO) in pig urine. The antigen CLO-OVA and goat anti-mouse IgG were attached to a nitrocellulose membrane as control line and test line. The cut-off limits of the test strips for CLO and ACLO were found to be 2.5 ng/mL in both 0.01 M PBS (pH 7.4) and pig urine. All the results were obtained within 5 min. The results revealed that the developed method is a sensitive, rapid, and simple tool for the detection of CLO and ACLO.

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