FCI: an R-based algorithm for evaluating uncertainty of absolute real-time PCR quantification
2008
Gallo Fabio | Pizzamiglio Sara | Verderio Paolo | Ramsden Simon C
<p>Abstract</p> <p>Background</p> <p>FCI is an R code for analyzing data from real-time PCR experiments. This algorithm estimates standard curve features as well as nucleic acid concentrations and confidence intervals according to Fieller's theorem.</p> <p>Results</p> <p>In order to describe the features of FCI four situations were selected from real data collected during an international external quality assessment program for quantitative assays based on real-time PCR. The code generates a diagnostic figure suitable for assessing the quality of the quantification process.</p> <p>Conclusion</p> <p>We have provided a freeware programme using this algorithm specifically designed to increase the information content of the real-time PCR assay.</p>
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