Classification of <i>Salmonella enterica</i> of the (Para-)Typhoid Fever Group by Fourier-Transform Infrared (FTIR) Spectroscopy
Miriam Cordovana | Norman Mauder | Markus Kostrzewa | Andreas Wille | Sandra Rojak | Ralf Matthias Hagen | Simone Ambretti | Stefano Pongolini | Laura Soliani | Ulrik S. Justesen | Hanne M. Holt | Olivier Join-Lambert | Simon Le Hello | Michel Auzou | Alida C. Veloo | Jürgen May | Hagen Frickmann | Denise Dekker
Typhoidal and para-typhoidal <i>Salmonella</i> are major causes of bacteraemia in resource-limited countries. Diagnostic alternatives to laborious and resource-demanding serotyping are essential. Fourier transform infrared spectroscopy (FTIRS) is a rapidly developing and simple bacterial typing technology. In this study, we assessed the discriminatory power of the FTIRS-based IR Biotyper (Bruker Daltonik GmbH, Bremen, Germany), for the rapid and reliable identification of biochemically confirmed typhoid and paratyphoid fever-associated <i>Salmonella</i> isolates. In total, 359 isolates, comprising 30 <i>S</i>. Typhi, 23 <i>S</i>. Paratyphi A, 23 <i>S</i>. Paratyphi B, and 7 <i>S</i>. Paratyphi C, respectively and other phylogenetically closely related <i>Salmonella</i> serovars belonging to the serogroups O:2, O:4, O:7 and O:9 were tested. The strains were derived from clinical, environmental and food samples collected at different European sites. Applying artificial neural networks, specific automated classifiers were built to discriminate typhoidal serovars from non-typhoidal serovars within each of the four serogroups. The accuracy of the classifiers was 99.9%, 87.0%, 99.5% and 99.0% for <i>Salmonella</i> Typhi, <i>Salmonella</i> Paratyphi A, B and <i>Salmonella</i> Paratyphi C, respectively. The IR Biotyper is a promising tool for fast and reliable detection of typhoidal <i>Salmonella</i>. Hence, IR biotyping may serve as a suitable alternative to conventional approaches for surveillance and diagnostic purposes.
Показать больше [+] Меньше [-]Библиографическая информация
Эту запись предоставил Directory of Open Access Journals
